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KMID : 1007520090180010249
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Food Science and Biotechnology
2009 Volume.18 No. 1 p.249 ~ p.252
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Stress Tolerance of Bifidobacterium infantis ATCC 27920 to Mild-heat Adaptation
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Kang Seo-Ku
Kim Young-Hoon
Cho In-Shick
Kang Ja-Heon
Chung Il-Byung
Kim Kwang-Hyun
Oh Se-Jong
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Abstract
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Two-dimensional gel electrophoresis (2-DE) was employed to assess the thermo-tolerance characteristics ofBifidobacterium infantis ATCC 27920 to mild heat adaptation. When exposed to various heat levels, pH, and hydrogen peroxide(H2O2) stress conditions, B. infantis ATCC 27920 exhibited high level of stress resistance. Under mild-heat treatment (46oC), nosignificant change in viability level was observed after 2 hr. Interestingly, improved viability was observed in mild-heat adapted(46oC for 1 hr) cultures exposed to 55oC, in comparison to control experiments. Viability was not affected by pH, bile, and H2O2stress conditions. 2-DE analysis revealed those mild-heat adaptation up-regulated 4 proteins and down-regulated 3 proteins.Among these protein spots, isopropylmalate dehydratase (leuD), glycosyltransferase (glgA), and ribosomal protein L5 (rplE) wereidentified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS).
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KEYWORD
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Bifidobacterium infants, thermotolerance, mild heat stress, 2-dimensional gel electrophoresis
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